We report a freely available software program, Pyromaker, which generates simulated traces for pyrosequencing results based on user inputs. Simulated pyrograms can aid in the analysis of complex ...

The pyrosequencing data analysis for EGFR, KRAS and BRAF is a manual process. Pyrosequencing data output is a pyrogram. The pyrogram consists of a series of peaks with different peak heights, which ...

Pyrosequencing-based methylation analysis investigates quantitatively the degree of methylation at CpG positions in close proximity after bisulfite treatment of genomic DNA. The procedure for a ...

Pyro Q-CpG from Biotage gives a new dimension to DNA methylation studies by quantitatively measuring the individual degree of methylation of consecutive CpG sites consistently over time. This reveals ...

(ii) Bead containing template on its surface is deposited in the picotiter microwell (iii) Immobilized enzymes (ATP sulphurylase, lucifearse, apyrase and DNA polymerase) are also deposited and ...

DNA sequencing to detect genetic mutations can aid in the diagnosis and selection of treatment for cancer. Current methods of testing DNA samples, Sanger sequencing and pyrosequencing, occasionally ...

Now, a team of Johns Hopkins researchers says it has developed an easy-to-use online computer software application that can clear up any confusion faster and cheaper than other methods currently used ...

Philadelphia, PA, February 6, 2012 - DNA sequencing to detect genetic mutations can aid in the diagnosis and selection of treatment for cancer. Current methods of testing DNA samples, Sanger ...